The effect of addition of melon (Cucumis melo L.) flesh juice into Lactated Ringer’s-egg yolk extender on spermatozoa plasma membrane integrity and spermatozoa morphological abnormalities of semen of native rooster

This study aims to determine the effect of adding melon (Cucumis melo L.) juice Lactated Ringer’s-egg yolk extender on intact plasma membrane (IPM) and morphological abnormalities native rooster spermatozoa. Five ejaculate each from two roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended 1:10 (v/v) in Ringer's-egg without or with addition 2, 4, 8% flesh juice, respectively for groups T0, T1, T2, T3. Extended semen stored a refrigerator (5°C) evaluated every hours. The results showed that IPM all decreased (p <0.05) consistently, lasting more than 40% six hours T0 T1 T2 T3 groups. percentage spermatozoa higher before storage. However, it not significantly different >0.05) which had been It concluded (group T0) 2% T1) at 5°C retained integrity less 20% While 4 % (T2) (T3) maintained only